Phylogenetic evidence for inter-typic recombination in the emergence of human enterovirus 71 subgenotypes

BMC Microbiology

Table 2 Oligonucleotide primers for cDNA synthesis and PCR.

Primer set	Primer	Nucleotide sequence (5' to 3')	Nucleotide position *	Polarity
EV1	EV1F	TAA AAC AGC YKK KGG KTK KYA CC	2–23	sense
	EV1R	TGM ACR TGR ATG CAR AAC C	1268–1286	antisense
EV2	EV2F	CCD GAT GTR YTR ACW GAA ACC	1200–1221	sense
	EV2R	ATC ACA TCT GCC ACY CTR TCY	2443–2463	antisense
EV3	EV3F	GGR GCR CCY AAY ACW GCY TAY ATW WTR GC	2333–2361	sense
	EV3R	CYT GRG CRG TRG TWG AHG AHA CKA RB	3451–3476	antisense
EV4	EV4F	CAR GTY TCH GTB CCR TTY ATG TCA CC	3005–3030	sense
	EV4R	GCT GTY TTB GMY TTR AYC CAV GC	4022–4044	antisense
EV5	EV5F	YAA CTC WMD SAG AAA RCA CTA YCC	3526–3559	sense
	EV5R	GWS GAK GCA ATS ACA AAC TTD GAK G	4719–4743	antisense
EV6	EV6F	CCW GAY CAY TTT GAY GGR TAY MAR C	4559–4583	sense
	EV6R	AHC CRT ACT GVV CVA CRT CHY C	5738–5759	antisense
EV7	EV7F	GTT GAG CGH CAC YTS AAY AGA GY	5216–5238	sense
	EV7R	GYA RRT CYA ACC CAT ACT TRT CC	6373–6395	antisense
EV8	EV8F	CAT YAA GAA RAR RGA CAT YYT BG	6310–6332	sense
	EV8R	TTG CTA TTC TGG TTA TAA CAA ATT TAC CC	7385–7413^#	antisense

ISSN: 1471-2180