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Table 2 Oligonucleotide primers for cDNA synthesis and PCR.

From: Phylogenetic evidence for inter-typic recombination in the emergence of human enterovirus 71 subgenotypes

Primer set Primer Nucleotide sequence (5' to 3') Nucleotide position * Polarity
EV1 EV1F TAA AAC AGC YKK KGG KTK KYA CC 2–23 sense
  EV1R TGM ACR TGR ATG CAR AAC C 1268–1286 antisense
EV2 EV2F CCD GAT GTR YTR ACW GAA ACC 1200–1221 sense
  EV2R ATC ACA TCT GCC ACY CTR TCY 2443–2463 antisense
EV3 EV3F GGR GCR CCY AAY ACW GCY TAY ATW WTR GC 2333–2361 sense
  EV3R CYT GRG CRG TRG TWG AHG AHA CKA RB 3451–3476 antisense
EV4 EV4F CAR GTY TCH GTB CCR TTY ATG TCA CC 3005–3030 sense
  EV4R GCT GTY TTB GMY TTR AYC CAV GC 4022–4044 antisense
EV5 EV5F YAA CTC WMD SAG AAA RCA CTA YCC 3526–3559 sense
  EV5R GWS GAK GCA ATS ACA AAC TTD GAK G 4719–4743 antisense
EV6 EV6F CCW GAY CAY TTT GAY GGR TAY MAR C 4559–4583 sense
  EV6R AHC CRT ACT GVV CVA CRT CHY C 5738–5759 antisense
EV7 EV7F GTT GAG CGH CAC YTS AAY AGA GY 5216–5238 sense
  EV7R GYA RRT CYA ACC CAT ACT TRT CC 6373–6395 antisense
EV8 EV8F CAT YAA GAA RAR RGA CAT YYT BG 6310–6332 sense
  EV8R TTG CTA TTC TGG TTA TAA CAA ATT TAC CC 7385–7413# antisense
  1. * The nucleotide position relative to the EV-71 MS87 strain
  2. # Include 2 positions in poly-A tail
  3. R = G/A Y = T/C M = A/C K = G/T S = G/C W = A/T
  4. H = not G B = not A V = not T D = not C