H-NS controls metabolism and stress tolerance in Escherichia coli O157:H7 that influence mouse passage

BMC Microbiology

Table 5 Plasmids and E. coli strains used in this study.

Strain or plasmid	Relevant characteristics^a	Source or reference
Strains
DH5α	supE44 ΔlacU169 (ϕ80 lacZ ΔM15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1	Lab collection
ATCC 43895	stx₁ and stx₂; other designation EDL 933W	Lab collection
SY327 λpir	Δ(lac pro) argE(Am) recA56 nalA Rif(λpir); π protein for R6K γ ori	[57]
SM10 λpir	thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu, λpir, oriT of RP4; Km^r	[57]
FRIK47001P^b	ATCC 43895, hns::nptI	This study
Plasmids
pBR322	Ap^r and Tc^r	Lab collection
pUC4K	pUC4 with nptI; Ap^r, Km^r	Pharmacia, [44]
pCVD442	R6K γ ori, sacB, oriT of RP4; Ap^r	J. Kaper, [45]
pSC9916	pBR322 containing Ap^r, Tc^s	[47]
pSC0061	pSC9916 containing hns; Tc^s, Ap^r	This study
pSC0062	pSC0061, hns::nptI	This study
pSC0063	pCVD442 containing hns::nptI	This study

^a Ap^r, ampicillin resistant; Ap^s, ampicillin sensitive; Km^r, kanamycin resistant; Tc^r, tetracycline resistant; Tc^s, tetracycline sensitive. Antibiotics were added to media when appropriate at the following concentrations: ampicillin 100 μg/ml, kanamycin 50 μg/ml, tetracycline 10 μg/ml.
^b FRIK47001P hns::nptI in the parallel orientation.

ISSN: 1471-2180