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Table 1 Growth of Escherichia coli O157:H7 strains in LB as influenced by pH, temperature, NaCl, and bile compounds.

From: H-NS controls metabolism and stress tolerance in Escherichia coli O157:H7 that influence mouse passage

 

Growth in LB

Growth conditions

ATCC 43895 (parent strain)

FRIK47001P (hns mutant)

FRIK47001P (pSC0061)

pHa

   

   37°C

4.0

4.5

4.0

   25°C

4.0

4.5

4.0

   16.5°C

4.0

5.0

4.0

NaCl (%)b

   

   37°C

6.0

5.0

6.0

   25°C

7.0

6.0

7.0

   16.5°C

6.0

4.0

4.0

Bile salts (%)c

14.0

6.5

14.0

Bile (%)d

15.0

9.0

15.0

  1. aMinimum pH at which growth occurred. LB adjusted to appropriate pH with 5 N HCl.
  2. bMaximum percent NaCl in LB at which growth occurred.
  3. cBile salts No. 3 (Difco). Maximum percent in LB that growth occurred at 37°C.
  4. dBovine bile (Sigma). Maximum percent in LB that growth occurred at 37°C.