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Table 3 Dilutions of primary and secondary antibodies for Western blotting

From: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system

Primary Antibody

Dilution

Secondary Antibody

Dilution

Source

Anti-MrgR

1:500

Swine Anti-rabbit IgG

1:2000

Bethyl Labs

Anti-MrgS

1:300

Swine Anti-rabbit IgG

1:1000

Bethyl Labs

Anti-MBP

1:1000

Swine Anti-rabbit IgG

1:2000

NEB

Convalescent sera pooled from 6 melioidosis patients

1:300

Rabbit Anti-human IgG

1:1000

Ty Pitt, HPA, London