Figure 1

Virtual normalization and direct subtraction. A) Relative signal intensity of clones, determined as a ratio of the signal intensity of each individual clone versus the maximum signal intensity present on the array. Signal intensities for the colonies derived from a single 384 well microplate are displayed as a function of relative colony signal strength. B) Proportion of unique ESTs obtained at various stages of the gene discovery process. 1: Sequencing of the first 192 randomly selected clones, 2: Sequencing of the last 192 clones of the 1920 randomly selected clones. 3: Sequencing of the first 192 clones obtained by virtual normalization. 4: Sequencing of the last 192 clones from the 2,304 clones obtained by virtual subtraction. 5: Sequencing of the first 192 clones selected after virtual normalization and direct subtraction, first round. 6: Sequencing of the last 192 clones selected after both virtual normalization and direct subtraction, first round. 7: Sequencing of the first 192 clones selected after virtual normalization and direct subtraction, second round. 8: Sequencing of the last 192 clones from the 10,738 clones selected after virtual normalization and direct subtraction, second round.