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Table 2 Genes essential for intracellular growth of F.tularensis SCHU S4 in Hep G2 cells

From: Identification of transposon insertion mutants of Francisella tularensis tularensis strain Schu S4 deficient in intracellular replication in the hepatic cell line HepG2

Strain Name Locus tag1/Genbank protein Accession # (*) Gene Name (putative function)
Nucleotide metabolism   
BJM1011 FTT1663/YP_170570 carA (carbamoyl-phosphate synthase small chain)
BJM1012 FTT1664/YP_170571.1 carB (carbamoyl-phosphate synthase large chain)
BJM1001 FTT1665/YP_170572.1 (2X) pyrB (aspartate carbamoyltransferase)
BJM1014 FTT0204/YP_169264.1 purA (adenylosuccinate synthetase)
BJM1015 FTT1720c/YP_170622.1 (3X) purL (phosphoribosylformylglycinamidine synthase)
BJM1018 FTT0203c/YP_169263.1 (3X) purH (bifunctional purine biosynthesis protein)
BJM1020 FTT1721c/YP_170623.1 (2X) purF (amidophosphoribosyltransferase)2
Amino acid transport and metabolism   
BJM1002 FTT0588/YP_169611.1 aroA (3-phosphoshikimate 1-carboxyvinyl transferase)
BJM1021 FTT1181c/YP_170144.1 ggt (gamma-glutamyl transpeptidase)
General function prediction   
BJM1003 FTT1244c/YP_170202.1 yfiO (conserved hypothetical lipoprotein)
BJM1022 FTT0129/YP_169196.1 major facilitator super family (MFS) transport protein
BJM1004 FTT0056c/YP_169131.1 major facilitator super family (MFS) transport protein
Inorganic ion transport metabolism   
BJM1024 FTT1490/YP_170425.1 Na+/H+ antiporter
Cell wall/membrane biogenesis   
BJM1023 FTT1234/YP_170192.1 choloylglycine hydrolase family protein
BJM1026 FTT1236/YP_170192.1 hypothetical protein
BJM1028 FTT1103/YP_170079.1 conserved hypothetical lipoprotein
Lipid metabolism   
BJM1013 FTT1762c/YP_170653.1 acetyltransferase protein
Posttranslational modification/protein turnover   
BJM1025 FTT1310c/YP_170263.1 hflB (ATP-dependent metalloprotease)
BJM1005 FTT0107c/YP_169177.1 (3X) dsbB (disulfide bond formation protein)
  1. 1Locus tag: http://biocyc.org/FRANT/
  2. 2This mutant was previously identified in F. tularensis novicida .
  3. (*) Number of times a mutant in this ORF was isolated [11].