Table 2 Genes essential for intracellular growth of F.tularensis SCHU S4 in Hep G2 cells

Nucleotide metabolism

BJM1011

FTT1663/YP_170570

carA (carbamoyl-phosphate synthase small chain)

BJM1012

FTT1664/YP_170571.1

carB (carbamoyl-phosphate synthase large chain)

BJM1001

FTT1665/YP_170572.1 (2X)

pyrB (aspartate carbamoyltransferase)

BJM1014

FTT0204/YP_169264.1

purA (adenylosuccinate synthetase)

BJM1015

FTT1720c/YP_170622.1 (3X)

purL (phosphoribosylformylglycinamidine synthase)

BJM1018

FTT0203c/YP_169263.1 (3X)

purH (bifunctional purine biosynthesis protein)

BJM1020

FTT1721c/YP_170623.1 (2X)

purF (amidophosphoribosyltransferase)²

Amino acid transport and metabolism

BJM1002

FTT0588/YP_169611.1

aroA (3-phosphoshikimate 1-carboxyvinyl transferase)

BJM1021

FTT1181c/YP_170144.1

ggt (gamma-glutamyl transpeptidase)

General function prediction

BJM1003

FTT1244c/YP_170202.1

yfiO (conserved hypothetical lipoprotein)

BJM1022

FTT0129/YP_169196.1

major facilitator super family (MFS) transport protein

BJM1004

FTT0056c/YP_169131.1

major facilitator super family (MFS) transport protein

Inorganic ion transport metabolism

BJM1024

FTT1490/YP_170425.1

Na+/H+ antiporter

Cell wall/membrane biogenesis

BJM1023

FTT1234/YP_170192.1

choloylglycine hydrolase family protein

BJM1026

FTT1236/YP_170192.1

hypothetical protein

BJM1028

FTT1103/YP_170079.1

conserved hypothetical lipoprotein

Lipid metabolism

BJM1013

FTT1762c/YP_170653.1

acetyltransferase protein

Posttranslational modification/protein turnover

BJM1025

FTT1310c/YP_170263.1

hflB (ATP-dependent metalloprotease)

BJM1005

FTT0107c/YP_169177.1 (3X)

dsbB (disulfide bond formation protein)