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Figure 7 | BMC Microbiology

Figure 7

From: Identification of transposon insertion mutants of Francisella tularensis tularensis strain Schu S4 deficient in intracellular replication in the hepatic cell line HepG2

Figure 7

Invasion and intracellular survival of BJM1001 and Schu S4 in HepG2 cells. HepG2 cells were infected with BJM1001 at an MOI to 50:1 in microtiter wells. After 2 hrs the media was replaced with media containing gentamicin. Sets of three wells were lysed at 3, 8, 24, 48 and 72 hrs, diluted, and spread on MHA plates to determine the number of intracellular colony forming units (CFUs). Each time point represents the average and standard deviation (S.D.) of three wells.

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