Figure 4From: Identification of transposon insertion mutants of Francisella tularensis tularensis strain Schu S4 deficient in intracellular replication in the hepatic cell line HepG2Southern blot of transposon insertion mutants of Schu S4 that were defective in intracellular growth in HepG2 cells. Chromosomal DNA from mutants digested with BclI was subjected Southern hybridization with a PCR product amplified from the EZ::TN<rpsLpRparr-2> DNA template. Lanes 1–15 contain DNA from rifampin-resistant mutants of Schu S4. Lane 16 contains wild-type Schu S4 DNA and Lane 17 contains the probe DNA. Each mutant contains a single copy of the transposon.Back to article page