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Figure 1 | BMC Microbiology

Figure 1

From: Identification of transposon insertion mutants of Francisella tularensis tularensis strain Schu S4 deficient in intracellular replication in the hepatic cell line HepG2

Figure 1

Map of transposon EZ::TN< rpsLpRparr-2 > that was used to create transposon-insertion library in Schu S4. Rparr-2 is a gene that encodes resistance to rifampin by inactivation of the drug. Arrows indicate the location of various primers that were used to for PCR amplification of the transposon or sequencing the flanking DNA. ME-mosaic ends (specifically recognized by the EZ::TN transposase), R6Kγori is an origin of replication that is used to rescue the transposon after it is inserted into the genome.

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