Table 1 Predicted functional motifs for ORF1-ORF14 of B. cenocepacia
From: Efflux pump genes of the resistance-nodulation-division family in Burkholderia cenocepacia genome
| Â | RND CONSERVED MOTIFS | FUNCTIONAL MOTIFS | STRUCTURAL ANALYSIS | |||
|---|---|---|---|---|---|---|
ORFsa | Motif s Ab, Bb, Cb, Db | Acriflavin resistance protein domains (ACRIFLAVINRP)c | AcrB transmembrane domainc | AcrB pore domain (PN1, PN2, PC1, PC2 subdomains)c | AcrB/TolC docking domain (DN, DC subdomains)c | Familyd |
BCAS0592 ORF1 Chromosome 3 | 38–134, 445–493, 826–1051, 272–503 | 8–32, 36–54, 336–359, 363–384, 391–415, 445–468, 470–493, 552–569, 620–634 | 272–503, 826–1051 | 38–134, 565–669 | 182–271, 720–825 | Multidrug efflux transporter AcrB pore domain |
BCAS0765 ORF2 Chromosome 3 | 38–134, 445–493, 809–1035, 272–503 | 8–32, 36–54, 336–359, 363–384, 391–415, 445–468, 470–493, 553–570, 622–636 | 272–503, 809–1035 | 38–134, 566–672 | 182–271, 723–808 | Multidrug efflux transporter AcrB transmembrane domain |
BCAL1675 ORF3 Chromosome 1 | 38–133, 444–492, 804–1027, 271–502 | 8–32, 36–54, 335–358, 362–383, 390–414, 444–467, 469–492, 551–568, 618–632 | 271–502, 804–1027 | 38–133, 564–668 | 719–803, 181–270 | Multidrug efflux transporter AcrB transmembrane domain |
BCAL2821 ORF4 Chromosome 1 | 38–134, 445–493, 809–1035, 272–503 | 8–32, 36–54, 336–359, 363–384, 391–415, 445–468, 470–493, 553–570, 622–636 | 272–503, 809–1035 | 38–134, 566–672 | 182–271, 723–808 | Multidrug efflux transporter AcrB transmembrane domain |
BCAL1778 ORF5 Chromosome 1 | 40–136, 446–494, 812–1037, 274–504 | 10–34, 38–56, 338–361, 365–386, 393–417, 446–469, 471–494, 562–579, 629–643 | 274–504, 812–1037 | 40–136, 575–676 | 184–273, 727–811 | Multidrug efflux transporter TolC docking domain |
BCAL1079 ORF6 Chromosome 1 | 40–136, 438–486, 792–1019, 266–496 | 10–34, 38–56, 330–353, 357–378, 385–409, 438–461, 463–486, 543–560, 610–624 | 266–496, 792–1019 | 40–136, 556–656 | 189–265, 707–791 | Multidrug efflux transporter AcrB pore domain |
BCAL1080 ORF7 Chromosome 1 | 40–134, 438–486, 853–1084, 266–496 | 10–34, 38–56, 330–353, 357–378, 385–409, 438–461, 463–486, 544–561, 607–621 | 266–496, 853–1084 | 40–134, 557–653 | 183–265, 704–776 | Multidrug efflux transporter AcrB pore domain |
BCAM0926 ORF8 Chromosome 2 | 38–134, 445–493, 803–1027, 272–503 | 8–32, 36–54, 336–359, 363–384, 391–415, 445–468, 470–493, 552–569, 619–633 | 272–503, 803–1027 | 38–134, 565–667 | 182–271, 718–802 | Multidrug efflux transporter AcrB pore domain |
BCAM1946 ORF9 Chromosome 2 | 40–136, 447–495, 814–1041, 275–505 | 10–34, 38–56, 339–362, 366–387, 394–418, 447–470, 472–495, 562–579, 631–645 | 275–505, 814–1041 | 40–136, 575–678 | 185–274, 729–813 | Multidrug efflux transporter TolC docking domain |
BCAM2550 ORF10 Chromosome 2 | 40–136, 447–495, 817–1043, 275–505 | 10–34, 38–56, 339–362, 366–387, 394–418, 447–470, 472–495, 562–579, 631–645 | 275–505, 817–1043 | 40–136, 575–681 | 185–274, 732–816 | Multidrug efflux transporter AcrB pore domain |
BCAM0713 ORF11 Chromosome 2 | 42–135, 480–503, 822–1049, 167–513 | 12–36, 40–58, 339–362, 366–387, 480–503, 553–570 | 167–513, 822–1049 | 42–135, 566–673 | 726–821 | Multidrug efflux transporter TolC docking domain |
BCAM0435 ORF12 Chromosome 2 | 42–135, 454–502, 807–1043, 269–511 | 12–36, 40–58, 334–357, 361–382, 389–413, 552–569 | 269–511, 807–1043 | 42–135, 565–610 | 185–268, 722–806 | Multidrug efflux transporter AcrB pore domain |
BCAL1811 ORF13 Chromosome 1 | 49–146, 446–494, 805–1029, 274–504 | 47–65, 339–362, 393–417, 446–469, 471–494, 554–571 | 274–504, 805–1029 | 49–146, 567–677 | 192–273, 721–804 | Multidrug efflux transporter AcrB pore domain |
BCAS0582 ORF14 Chromosome 3 | 40–136, 438–486, 832–1060, 266–496 | 10–34, 38–56, 330–353, 357–378, 385–409, 438–461, 463–486, 551–568 | 266–496, 832–1060 | 40–136, 564–675 | 183–265, 727–831 | Multidrug efflux transporter AcrB pore domain |