Figure 2

Expression of genes coding for the RND proteins from B. cenocepacia. Expression of genes coding for the RND proteins was evaluated by RT-PCR of RNA isolated from B. cenocepacia cultures grown in the LB standard medium without and with chloramphenicol. A) Lane M represents the molecular weight marker (Fermentas), whose sizes of some bands in base pairs (bp) are provided on the left; lanes 3, 5, 7, 9, and 11 represent positive controls, i.e. genomic DNA amplification with primers specific for orf3, orf9, orf10, orf11, and orf13 (Table 3); lanes 1 and 2 represent RT-PCR amplification of orf3 in the absence and in the presence of 12.8 μg/ml (0.4X MIC) of chloramphenicol, respectively; lanes 4, 6, 8, and 10 represent RT-PCR amplifications of orf9, orf10, orf11, and orf13, without antibiotic, respectively; lane CT represents the PCR reaction negative control. B) Lanes 1 and 2 represent the expression of rRNA 16S gene in the presence or in the absence of chloramphenicol, respectively; lane 3 represents the positive control, i.e. genomic DNA amplification with primers specific for rRNA 16S gene; lane M represents the molecular weight marker (Fermentas), whose sizes of some bands in base pairs (bp) are provided on the right.