Table 3 Strains and plasmids used in this study

MC4100

F^-ΔlacU169 araD139 rpsL150 relA1 ptsF rbs flbB5301

[58]

DH5α

φ80dlac ZΔM15, recA1, endA1, gyrA96, thi-1, hsdR 17(rK^-,mK+) supE44, relA1, deoR, Δ(lacZYA-argF) U169

Promega

M15

F^-, lac, ara, gal, mtl

[59]

JARV16-P

As MC4100 ΔtatA ΔtatE pcn B1 zad-981::Tn10 d (Km^r)

[8]

BØD-P

As MC4100 ΔtatB pcn B1 zad-981::Tn10 d (Km^r)

[8]

B1LK0-P

As MC4100 ΔtatC pcn B1 zad-981::Tn10 d (Km^r)

This work

DADE-P

As MC4100 ΔtatABCD ΔtatE pcn B1 zad-981::Tn10 d (Km^r)

[60]

Plasmids

pREP4

Kan^R, lacI⁺

Roche

pBluescript (IIKS⁺)

Amp^R

Stratagene

pFAT415

pBluescript carrying E. coli tatA

[8]

pFAT416

pBluescript carrying E. coli tatB

[8]

pFAT417

pBluescript carrying E. coli tatC

[8]

pUniprom

Amp^R contains E. coli tat promoter and multiple cloning site

[51]

pUniprom-PA

pUniprom carrying P. syringae tatA gene

This work

pUniprom-PB

pUniprom carrying P. syringae tatB gene

This work

pUniprom-PC

pUniprom carrying P. syringae tatC gene

This work

pUniprom-PABC

pUniprom carrying P. syringae tatABC genes

This work

pUniprom-SA

pUniprom carrying S. coelicolor tatA gene

This work

pUniprom-SB

pUniprom carrying S. coelicolor tatB gene

This work

pUniprom-SC

pUniprom carrying S. coelicolor tatC gene

This work

pFATAQ1

pBluescript carrying A. aeolicus tatA1 and tatA2

This work

pFATAQ2

pBluescript carrying A. aeolicus tatBC

This work

pFATAQ3

pBluescript carrying A. aeolicus tatA, tatA2 and tatBC

This work

pQE70

C-terminal His-tag expression vector

Qiagen

pQEAQ1

pQE70 carrying A. aeolicus tatA1

This work

pQEAQ2

pQE70 carrying A. aeolicus tatA2

This work

pQEAQBC

pQE70 carrying A. aeolicus tatBC

This work

pQEAQ12

pQE70 carrying A. aeolicus tatA1 and tatA2

This work