Formation of functional Tat translocases from heterologous components

BMC Microbiology

Table 2 Summary of Tat activity observed after heterologous expression of tat genes from different bacteria in E. coli tat mutant strains.

E. coli strain	Plasmid-encoded gene(s)	Functionality of the Tat system¹
JARV16-P(ΔtatA/ΔtatE)	Eco tatA	++
	Psy tatA	++
	Sco tatA	++
	Sco tatA(L₄₀F)	++
	Aae tatA1	+
	Aae tatA2	+
BØD-P(ΔtatB)	Eco tatB	++
	Psy tatB	+
	Sco tatB	++
	Aae tatB	-
B1LK0-P(ΔtatC)	Eco tatC	++
	Psy tatC	-
	Sco tatC	++
	Aae tatC	-
DADE-P(ΔtatABCD/ΔtatE)	Eco tatABC	++
	Psy tatABC	+
	Aae tatA1A2tatBC	-

¹++ indicates a high level of functionality (>30% of the wild type level of periplasmic TMAO reductase activity, + indicates a lower level of functionality (<30% of the wild type level of periplasmic TMAO reductase activity, but confers the ability to grow on solid media containing SDS or TMAO), – indicates a lack of activity (no growth on SDS or TMAO-containing plates).

ISSN: 1471-2180