Skip to main content

Advertisement

Table 2 Summary of Tat activity observed after heterologous expression of tat genes from different bacteria in E. coli tat mutant strains.

From: Formation of functional Tat translocases from heterologous components

E. coli strain Plasmid-encoded gene(s) Functionality of the Tat system1
JARV16-P(ΔtatAtatE) Eco tatA ++
  Psy tatA ++
  Sco tatA ++
  Sco tatA(L40F) ++
  Aae tatA1 +
  Aae tatA2 +
BØD-P(ΔtatB) Eco tatB ++
  Psy tatB +
  Sco tatB ++
  Aae tatB -
B1LK0-P(ΔtatC) Eco tatC ++
  Psy tatC -
  Sco tatC ++
  Aae tatC -
DADE-P(ΔtatABCDtatE) Eco tatABC ++
  Psy tatABC +
  Aae tatA1A2tatBC -
  1. 1++ indicates a high level of functionality (>30% of the wild type level of periplasmic TMAO reductase activity, + indicates a lower level of functionality (<30% of the wild type level of periplasmic TMAO reductase activity, but confers the ability to grow on solid media containing SDS or TMAO), – indicates a lack of activity (no growth on SDS or TMAO-containing plates).