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Figure 4 | BMC Microbiology

Figure 4

From: Formation of functional Tat translocases from heterologous components

Figure 4

Complementation of the E. coli ΔtatABCD ΔtatE, pcnB strain, DADE-P, with tatABC genes from other bacteria. Strain DADE-P was transformed with either: E. coli tatABC encoded on pUnitat2 (EcoABC), P. syringae tatABC on plasmid pUniprom-PABC (PsyABC), A. aeolicus tatA1A2BC from plasmid pFATAQ3 (AaeA1A2BC) or pBluescript (empty vector; marked as Δ in panel C). A. Growth of strains on LB medium containing 2% SDS. B. Growth of strains anaerobically on minimal glycerol TMAO medium. C. TMAO reductase activities from periplasmic fractions. *100% activity is taken as that determined from the periplasmic fraction of DADE-P carrying pUnitat2 and corresponds to 0.83μmol benzyl viologen oxidised/min/mg protein. Error bars represent the standard error of the mean (n = 3).

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