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Table 2 ALO-mediated lysis of human monocytes by B. anthracis is inhibited by 10% FBS. Percent viable THP-1s in the presence of B. anthracis Sterne strain 7702 (ALO+), UT231 (ALO-) and the complemented over-producer, UT231 (pUTE544) (ALO++) at an MOI of 1000 in the presence of 10 % FBS. At various time points, aliquots were mixed with 0.25 % trypan blue to measure viability. Native ALO expressed by 7702, at all time points, and UT231 (pUTE544), at 5 min, is inhibited by cholesterol present in heat-inactivated 10% FBS. Results are average ± standard deviations of two experiments.

From: The Bacillus anthracis cholesterol-dependent cytolysin, Anthrolysin O, kills human neutrophils, monocytes and macrophages

% Viable THP-1 monocytes in presence of 10% FBS
  Sterne 7702 UT231 UT231(pUTE544) Control (no bacteria)
5 min 100 100 100 100
30 min 100 100 0 99 ± 0
60 min 95 ± 1 98 ± 2 0 99 ± 0
120 min 92 ± 3.2 96 ± 1 0 97 ± 1