Figure 6From: The Bacillus anthracis cholesterol-dependent cytolysin, Anthrolysin O, kills human neutrophils, monocytes and macrophagesCell free supernatants from B. anthracis secreting ALO lyse human monocyte derived macrophages significantly more than the ALO knockout strain, UT231, in a dose dependent manner. Percent viability of hMDMs from three different donors measured by trypan blue after the addition of filter sterilized B. anthracis supernatants in presence of heat-inactivated 10% FBS. The 3 h time point is shown, as little lysis was observed at earlier time points. Results are representative of results from three different experiments. (Dose, p < 0.001, strain, p < 0.001, dose by strain interaction, p = 0.044, as calculated by 2-way ANOVA. Tukey test showing significant differences between UT231/7702, UT231/UT231(pUTE544), 7702/UT231(pUTE544), p < 0.001)Back to article page