Figure 5From: The Bacillus anthracis cholesterol-dependent cytolysin, Anthrolysin O, kills human neutrophils, monocytes and macrophagesHemolytic activity of BHI growth supernatants of B. anthracis strains 7702, the parent ALO+ strain, and UT231(pUTE544), the ALO knockout strain complemented in trans with a plasmid bearing the alo gene. UT231 (pUTE544) produces about 1,000-fold more ALO. Hemolytic activity is reduced upon the addition of 1 μg/ml cholesterol. Results are representative of at least three independent experiments done in duplicate.Back to article page