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Figure 4 | BMC Microbiology

Figure 4

From: The Bacillus anthracis cholesterol-dependent cytolysin, Anthrolysin O, kills human neutrophils, monocytes and macrophages

Figure 4

Recombinant ALO lyses primary human lymphocytes in a dose dependent manner. Percent viability of lymphocytes measured with trypan blue after 30 min incubation with varying concentrations of rALO at 37°C with no serum or heat-inactivated 10%. The means and standard deviations of at least three independent experiments, each done in duplicate, are shown (Spearman correlation is -0.964, p < 0.001). Addition of 10% serum significantly inhibits ALO-mediated lymphocyte lysis (*p = 0.001 as calculated by student's unpaired t-test).

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