Figure 3

Recombinant ALO lyses human monocyte derived macrophages (hMDMs) in a dose dependent manner. Percent viability of primary human monocyte derived macrophages after 30 min incubation at 37°C with varying concentrations of rALO in serum free conditions. The means and standard deviations of three independent experiments performed in duplicate are shown. Each experiment was performed with hMDMs from different donors (represented by ▲, ◆, ■) (▲, Spearman correlation is -0.917, p = 0.001. ◆, Spearman correlation is -0.962, p < 0.001. ■, Spearman correlation is -0.852, p = 0.015).