Figure 2

Recombinant ALO lyses human monocytic THP-1 cells in a dose-dependent manner. Percent viability of THP-1 monocytes measured by trypan blue after 30 min incubation with various concentrations of rALO in serum free conditions. Results show that 50% of THP-1s are lysed by 0.35 nM of rALO. The means and standard deviations of at least three independent experiments, each performed in duplicate, are shown (Spearman correlation is -0.870, p = 0.002).