Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas testosteroni in mixed microbial communities

Table 1 PCR primers and FISH probes used in this study

Name	Sequence 5'-3'	T_M/FA^a	Position^b	Reference
CteA1-for	CGAAAAGCCTGGGGCTAATAT	58°C	449-469	this study
CteA1-rev	CCATCTCTGGTAAGTTCCTGC	60°C	1019-999	this study
CteA2-for	TTGACATGGCAGGAACTTACC	58°C	991-1011	this study
CteA2-rev	TCCCATTAGAGTGCTCAACTG	58°C	1157-1136	this study
341F	CCTACGGGAGGCAGCAG	60°C	341-356	[14]
534R	ATTACCGCGGCTGCTGGC	60°C	534-517	[14]
27F	AGAGTTTGATCATGGCTCAG	55°C	8-27	[35]
1492R	GGTTACCTTGTTACGACTT	53°C	1510-1492	[35]
CteA	CATGACCCGGGGATATTAGC	30%	481-462	this study
Eub338	GCTGCCTCCCGTAGGAGT	30%	355-338	[38]
Eub338-II	GCAGCCACCCGTAGGTGT	30%	355-338	[39]
Eub338-III	GCTGCCACCCGTAGGTGT	30%	355-338	[39]

^a Melting temperature for PCR primers or formamide concentrations for FISH probes, respectively
^b Position on the E. coli 16S rRNA sequence

ISSN: 1471-2180