From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules
Strains/plasmids/phage | Relevant genotype | Source/reference |
---|---|---|
Strains* | Â | Â |
GS162 | WT | This laboratory |
GS1053 | gcvR::Tn10 | [19] |
GS1118 | ΔgcvA:ΣaadA | This laboratory |
GS1131 | ΔgcvA:ΣaadA ΔgcvR:ΣKNR | [11] |
GS1132 | Δ(gcvA gcvB):ΣaadA | [11] |
GS1144 | ΔgcvB:ΣCMR | This laboratory |
KIM6 | lcr - | [31] |
KIM6ΔgcvB | ΔgcvB:ΣCMR | This study |
Plasmid | Â | Â |
pGS366 | Single-copy translational lacZ fusion vector | This laboratory |
pgcvBYp-p322 | Carries Y. pestis gcvB in pBR322 | This study |
pgcvBYp-sc | Carries Y. pestis gcvB in a single-copy vector | This study |
pgcvAYp-p177 | Carries Y. pestis gcvA in pACYC177 | This study |
pgcvRYp-p177 | Carries Y. pestis gcvR in pACYC177 | This study |
pgcvRYp-p322 | Carries Y. pestis gcvR in pBR322 | This study |
pdppAYp::lacZ | Y. pestis dppA::lacZ fusion in pGS366 | This study |
pGS335 | Carries E. coli gcvA in pACYC177 | This lab |
pGS601 | Carries E. coli gcvR in pACYC177 | This lab |
Phage | Â | Â |
λdppA::lacZ | λgt2 with E. coli dppA::lacZ translational fusion | [11] |
λgcvB::lacZ | λgt2 with E. coli gcvB+50::lacZ transcriptional fusion | [11] |
λgcvBYp+53::lacZ | λgt2 with Y. pestis gcvB+53::lacZ transcriptional fusion | This study |
λgcvBYp+164::lacZ | λgt2 with Y. pestis gcvB+164::lacZ transcriptional fusion | This study |
λgcvBYp+251::lacZ | λgt2 with Y. pestis gcvB+251::lacZ transcriptional fusion | This study |