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Table 4 The Y. pestis gcvR gene complements an E. coli gcvR mutation. Cells were grown in GM media to an OD600 of ~0.5 and assayed for β-galactosidase activity [16]. Activity is expressed in Miller units.

From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules

Lysogen Relevant genotype β-Galactosidase activity
GS1053λgcvB::lacZ GcvR 308 ± 19
GS1053λgcvB::lacZ[pACYC177] GcvR 384 ± 175
GS1053λgcvB::lacZ[pGS601] gcvR/gcvR Ec 11 ± 1.5
GS1053λgcvB::lacZ[pgcvRYp-p177] gcvR/gcvRYp 14 ± 1.2
GS1131λgcvB::lacZ ΔgcvA ΔgcvR 2.2 ± 0.2
GS1131λgcvB::lacZ[pgcvRYp-p322] ΔgcvA ΔgcvR/gcvRYp 2.8 ± 0.1
GS1131λgcvB::lacZ[pgcvAYp-p177] ΔgcvA ΔgcvR/gcvAYp 393 ± 8
GS1131λgcvB::lacZ[pgcvAYp-177 pgcvRYp-p322] ΔgcvA ΔgcvR/gcvAYpgcvRYp 6.8 ± 0.4