Table 4 The Y. pestis gcvR gene complements an E. coli gcvR mutation. Cells were grown in GM media to an OD600 of ~0.5 and assayed for β-galactosidase activity [16]. Activity is expressed in Miller units.
From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules
Lysogen | Relevant genotype | β-Galactosidase activity |
|---|---|---|
GS1053λgcvB::lacZ | GcvR | 308 ± 19 |
GS1053λgcvB::lacZ[pACYC177] | GcvR | 384 ± 175 |
GS1053λgcvB::lacZ[pGS601] | gcvR/gcvR Ec | 11 ± 1.5 |
GS1053λgcvB::lacZ[pgcvRYp-p177] | gcvR/gcvRYp | 14 ± 1.2 |
GS1131λgcvB::lacZ | ΔgcvA ΔgcvR | 2.2 ± 0.2 |
GS1131λgcvB::lacZ[pgcvRYp-p322] | ΔgcvA ΔgcvR/gcvRYp | 2.8 ± 0.1 |
GS1131λgcvB::lacZ[pgcvAYp-p177] | ΔgcvA ΔgcvR/gcvAYp | 393 ± 8 |
GS1131λgcvB::lacZ[pgcvAYp-177 pgcvRYp-p322] | ΔgcvA ΔgcvR/gcvAYpgcvRYp | 6.8 ± 0.4 |