Skip to main content

Table 2 Regulation of the Y. pestis gcvB+53::lacZ transcriptional fusion in E. coli. Cells were grown in GM media with the indicated supplements to an OD600 of ~0.5 and assayed for β-galactosidase activity [16]. Activity is expressed in Miller units.

From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules

Lysogen Relevant genotype β-galactosidase activity for cells grown in:
   GM GM + glycine GM + inosine
GS162λgcvBYp+53::lacZ WT 15 ± 2 173 ± 2 6 ± 3
GS1118λgcvBYp+53::lacZ ΔgcvA 2 ± 1 3 ± 1 2 ± 1
GS1053λgcvBYp+53::lacZ gcvR 620 ± 58 419 ± 13 440 ± 174
GS1144λgcvBYp+53::lacZ ΔgcvB 10 ± 2 140 ± 24 6 ± 2