|
C. perfringens
| | |
|
SM101
|
Electroporatable derivative of food poisoning type A isolate, NCTC8798, carries a chromosomal cpe
|
[28]
|
|
NCTC8239
|
Food poisoning type A isolate carrying a chromosomal cpe
|
[30]
|
|
FD1041
|
Food poisoning type A isolate carrying a chromosomal cpe
|
[30]
|
|
NCTC10239
|
Food poisoning type A isolate carrying a chromosomal cpe
|
[30]
|
|
C1841
|
Food poisoning type A isolate carrying a chromosomal cpe
|
[30]
|
|
13
|
Type A, laboratory strain
|
[18]
|
|
IH101
|
spo0A mutant derivative of SM101
|
[19]
|
|
DR101
|
ssp3 mutant derivative of SM101
|
This study
|
|
Plasmids
| | |
|
pJIR 751
|
C. perfringens – E. coli shuttle vector ; Emr
|
[25]
|
|
pJIR 418
|
C. perfringens – E. coli shuttle vector ; Cmr, Emr
|
[31]
|
|
pMRS104
|
Suicidal plasmid, no origin of replication for Clostridia; Emr
|
[19]
|
|
pSM104
|
cpe-gusA in pJIR750; Cmr
|
[26]
|
|
pSM242
|
sigK-gusA fusion in pJIR750; Cmr
|
S.B. Melville
|
|
pMRS127
|
sigK-gusA fusion in pJIR751; Emr
|
This study
|
|
pMRS130
|
pJIR751 carrying promoter-less gusA; Emr
|
This study
|
|
pBH2
|
ssp3 and 1.9-kb each upstream and downstream region cloned into pCR®-XL-TOPO®.
|
This study
|
|
pSG11
|
ssp1 promoter in pCR®-XL-TOPO®
|
This study
|
|
pSG12
|
ssp1-gusA in pMRS127; Emr
|
This study
|
|
pDR13
|
ssp2 and 1.4-kb each upstream and downstream region cloned into pCR®-XL-TOPO®.
|
This study
|
|
pDR14
|
Nru I site incorporated into ssp2 in pDR13
|
This study
|
|
pDR17
|
ssp3 with its promoter in pCR®-XL-TOPO®
|
This study
|
|
pDR18
|
ssp3 with its promoter in pJIR751; Emr
|
This study
|
|
pSG21
|
ssp2 promoter in pCR®-XL-TOPO®
|
This study
|
|
pSG22
|
ssp2-gusA in pMRS127; Emr
|
This study
|
|
pDR26
|
ssp2::catP in pCR®-XL-TOPO®
|
This study
|
|
pDR27
|
ssp2::catP in pMRS104; EmrCmr
|
This study
|
|
pSG31
|
ssp3 promoter in pCR®-XL-TOPO®
|
This study
|
|
pDR 31
|
ssp1 and 1.5-kb each upstream and downstream region cloned into pCR®-XL-TOPO®.
|
This study
|
|
pSG32
|
ssp3-gusA in pMRS127; Emr
|
This study
|
|
pMRS60
|
Hpa I site incorporated into ssp3 in pBH2
|
This study
|
|
pMRS61
|
ssp3::catP in pMRS60; CmrKmr
|
This study
|
|
pMRS62
|
ssp3::catP in pMRS104; EmrCmr
|
This study
|
|
pDR61
|
Nru I site incorporated into ssp1 in pDR31
|
This study
|
|
pDR62
|
ssp1::catP in pDR61; CmrKmr
|
This study
|
|
pDR63
|
ssp1::catP in pMRS104; EmrCmr
|
This study
|
