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Table 1 Bacterial strains and Plasmids used in this study

From: Investigating the role of small, acid-soluble spore proteins (SASPs) in the resistance of Clostridium perfringens spores to heat

Bacterial strain/Plasmid Relevant Characteristicsa Sources or Reference
C. perfringens   
   SM101 Electroporatable derivative of food poisoning type A isolate, NCTC8798, carries a chromosomal cpe [28]
   NCTC8239 Food poisoning type A isolate carrying a chromosomal cpe [30]
   FD1041 Food poisoning type A isolate carrying a chromosomal cpe [30]
   NCTC10239 Food poisoning type A isolate carrying a chromosomal cpe [30]
   C1841 Food poisoning type A isolate carrying a chromosomal cpe [30]
   13 Type A, laboratory strain [18]
   IH101 spo0A mutant derivative of SM101 [19]
   DR101 ssp3 mutant derivative of SM101 This study
Plasmids   
   pJIR 751 C. perfringensE. coli shuttle vector ; Emr [25]
   pJIR 418 C. perfringensE. coli shuttle vector ; Cmr, Emr [31]
   pMRS104 Suicidal plasmid, no origin of replication for Clostridia; Emr [19]
   pSM104 cpe-gusA in pJIR750; Cmr [26]
   pSM242 sigK-gusA fusion in pJIR750; Cmr S.B. Melville
   pMRS127 sigK-gusA fusion in pJIR751; Emr This study
   pMRS130 pJIR751 carrying promoter-less gusA; Emr This study
   pBH2 ssp3 and 1.9-kb each upstream and downstream region cloned into pCR®-XL-TOPO®. This study
   pSG11 ssp1 promoter in pCR®-XL-TOPO® This study
   pSG12 ssp1-gusA in pMRS127; Emr This study
   pDR13 ssp2 and 1.4-kb each upstream and downstream region cloned into pCR®-XL-TOPO®. This study
   pDR14 Nru I site incorporated into ssp2 in pDR13 This study
   pDR17 ssp3 with its promoter in pCR®-XL-TOPO® This study
   pDR18 ssp3 with its promoter in pJIR751; Emr This study
   pSG21 ssp2 promoter in pCR®-XL-TOPO® This study
   pSG22 ssp2-gusA in pMRS127; Emr This study
   pDR26 ssp2::catP in pCR®-XL-TOPO® This study
   pDR27 ssp2::catP in pMRS104; EmrCmr This study
   pSG31 ssp3 promoter in pCR®-XL-TOPO® This study
   pDR 31 ssp1 and 1.5-kb each upstream and downstream region cloned into pCR®-XL-TOPO®. This study
   pSG32 ssp3-gusA in pMRS127; Emr This study
   pMRS60 Hpa I site incorporated into ssp3 in pBH2 This study
   pMRS61 ssp3::catP in pMRS60; CmrKmr This study
   pMRS62 ssp3::catP in pMRS104; EmrCmr This study
   pDR61 Nru I site incorporated into ssp1 in pDR31 This study
   pDR62 ssp1::catP in pDR61; CmrKmr This study
   pDR63 ssp1::catP in pMRS104; EmrCmr This study
  1. a. Emr, Cmr, Kmr: resistance to erythromycin, chloramphenicol and kanamycin, respectively.