Skip to main content

Table 1 Bacterial strains and Plasmids used in this study

From: Investigating the role of small, acid-soluble spore proteins (SASPs) in the resistance of Clostridium perfringens spores to heat

Bacterial strain/Plasmid

Relevant Characteristicsa

Sources or Reference

C. perfringens

  

   SM101

Electroporatable derivative of food poisoning type A isolate, NCTC8798, carries a chromosomal cpe

[28]

   NCTC8239

Food poisoning type A isolate carrying a chromosomal cpe

[30]

   FD1041

Food poisoning type A isolate carrying a chromosomal cpe

[30]

   NCTC10239

Food poisoning type A isolate carrying a chromosomal cpe

[30]

   C1841

Food poisoning type A isolate carrying a chromosomal cpe

[30]

   13

Type A, laboratory strain

[18]

   IH101

spo0A mutant derivative of SM101

[19]

   DR101

ssp3 mutant derivative of SM101

This study

Plasmids

  

   pJIR 751

C. perfringensE. coli shuttle vector ; Emr

[25]

   pJIR 418

C. perfringensE. coli shuttle vector ; Cmr, Emr

[31]

   pMRS104

Suicidal plasmid, no origin of replication for Clostridia; Emr

[19]

   pSM104

cpe-gusA in pJIR750; Cmr

[26]

   pSM242

sigK-gusA fusion in pJIR750; Cmr

S.B. Melville

   pMRS127

sigK-gusA fusion in pJIR751; Emr

This study

   pMRS130

pJIR751 carrying promoter-less gusA; Emr

This study

   pBH2

ssp3 and 1.9-kb each upstream and downstream region cloned into pCR®-XL-TOPO®.

This study

   pSG11

ssp1 promoter in pCR®-XL-TOPO®

This study

   pSG12

ssp1-gusA in pMRS127; Emr

This study

   pDR13

ssp2 and 1.4-kb each upstream and downstream region cloned into pCR®-XL-TOPO®.

This study

   pDR14

Nru I site incorporated into ssp2 in pDR13

This study

   pDR17

ssp3 with its promoter in pCR®-XL-TOPO®

This study

   pDR18

ssp3 with its promoter in pJIR751; Emr

This study

   pSG21

ssp2 promoter in pCR®-XL-TOPO®

This study

   pSG22

ssp2-gusA in pMRS127; Emr

This study

   pDR26

ssp2::catP in pCR®-XL-TOPO®

This study

   pDR27

ssp2::catP in pMRS104; EmrCmr

This study

   pSG31

ssp3 promoter in pCR®-XL-TOPO®

This study

   pDR 31

ssp1 and 1.5-kb each upstream and downstream region cloned into pCR®-XL-TOPO®.

This study

   pSG32

ssp3-gusA in pMRS127; Emr

This study

   pMRS60

Hpa I site incorporated into ssp3 in pBH2

This study

   pMRS61

ssp3::catP in pMRS60; CmrKmr

This study

   pMRS62

ssp3::catP in pMRS104; EmrCmr

This study

   pDR61

Nru I site incorporated into ssp1 in pDR31

This study

   pDR62

ssp1::catP in pDR61; CmrKmr

This study

   pDR63

ssp1::catP in pMRS104; EmrCmr

This study

  1. a. Emr, Cmr, Kmr: resistance to erythromycin, chloramphenicol and kanamycin, respectively.