Table 1 Bacterial strains and Plasmids used in this study
Bacterial strain/Plasmid | Relevant Characteristicsa | Sources or Reference |
|---|---|---|
C. perfringens | ||
SM101 | Electroporatable derivative of food poisoning type A isolate, NCTC8798, carries a chromosomal cpe | [28] |
NCTC8239 | Food poisoning type A isolate carrying a chromosomal cpe | [30] |
FD1041 | Food poisoning type A isolate carrying a chromosomal cpe | [30] |
NCTC10239 | Food poisoning type A isolate carrying a chromosomal cpe | [30] |
C1841 | Food poisoning type A isolate carrying a chromosomal cpe | [30] |
13 | Type A, laboratory strain | [18] |
IH101 | spo0A mutant derivative of SM101 | [19] |
DR101 | ssp3 mutant derivative of SM101 | This study |
Plasmids | ||
pJIR 751 | C. perfringens – E. coli shuttle vector ; Emr | [25] |
pJIR 418 | C. perfringens – E. coli shuttle vector ; Cmr, Emr | [31] |
pMRS104 | Suicidal plasmid, no origin of replication for Clostridia; Emr | [19] |
pSM104 | cpe-gusA in pJIR750; Cmr | [26] |
pSM242 | sigK-gusA fusion in pJIR750; Cmr | S.B. Melville |
pMRS127 | sigK-gusA fusion in pJIR751; Emr | This study |
pMRS130 | pJIR751 carrying promoter-less gusA; Emr | This study |
pBH2 | ssp3 and 1.9-kb each upstream and downstream region cloned into pCR®-XL-TOPO®. | This study |
pSG11 | ssp1 promoter in pCR®-XL-TOPO® | This study |
pSG12 | ssp1-gusA in pMRS127; Emr | This study |
pDR13 | ssp2 and 1.4-kb each upstream and downstream region cloned into pCR®-XL-TOPO®. | This study |
pDR14 | Nru I site incorporated into ssp2 in pDR13 | This study |
pDR17 | ssp3 with its promoter in pCR®-XL-TOPO® | This study |
pDR18 | ssp3 with its promoter in pJIR751; Emr | This study |
pSG21 | ssp2 promoter in pCR®-XL-TOPO® | This study |
pSG22 | ssp2-gusA in pMRS127; Emr | This study |
pDR26 | ssp2::catP in pCR®-XL-TOPO® | This study |
pDR27 | ssp2::catP in pMRS104; EmrCmr | This study |
pSG31 | ssp3 promoter in pCR®-XL-TOPO® | This study |
pDR 31 | ssp1 and 1.5-kb each upstream and downstream region cloned into pCR®-XL-TOPO®. | This study |
pSG32 | ssp3-gusA in pMRS127; Emr | This study |
pMRS60 | Hpa I site incorporated into ssp3 in pBH2 | This study |
pMRS61 | ssp3::catP in pMRS60; CmrKmr | This study |
pMRS62 | ssp3::catP in pMRS104; EmrCmr | This study |
pDR61 | Nru I site incorporated into ssp1 in pDR31 | This study |
pDR62 | ssp1::catP in pDR61; CmrKmr | This study |
pDR63 | ssp1::catP in pMRS104; EmrCmr | This study |