Production of SASPs by cpe-positive C. perringens. (A) SASPs, prepared from a representative C. perfringens food poisoning isolate SM101, was separated by polyacrylamide gel electrophoresis at low pH and stained with Coomassie brilliant blue. (B) Western blot of the gel shown in panel A. The blot was probed with antibodies against B. subtilis SspC  and developed by chemiluminescence detection to identify immunoreactive species. Arrow indicates the major band that was immunoreactive with B. subtilis SspC antibody.