Inhibition of trophozoite proliferation by a PI3K inhibitor. a) Trophozoites treated with increasing concentrations of LY294002 were counted after 48 hours of treatment. b) To test the specificity of inhibition, the effect of LY294002 (50 μM) and DRB (100 μM final concentration) on exponentially growing trophozoites was monitored by cell counting over a 48 hour period. Error bars indicate the standard deviation of 3 counts. Asterisks denote significance at p < 0.001 for normalised data.