Acanthamoeba (T1) proteases degrade various protein substrates including extracellular matrix (ECM). Zymographic assays were performed using various substrates that constitute ECM or involved in ECM degradation. Note that proteases of AGE isolate degraded collagen I (A), collagen III (B), elastin (C) and plasminogen (F). In addition, both proteases degraded gelatin (D), casein (E) and haemoglobin (G). CM is conditioned medium plus or minus the serine protease inhibitor, PMSF. Results are representative of three independent experiments.