DNA used as template | Species detected |
|---|
F. graminearum
|
F. culmorum
|
F. graminearum
|
F. culmorum
|
|---|
5 pg | 5 pg |
+
|
+
|
100 pg | 100 pg |
-
|
+
|
100 pg | 10 pg |
+
|
+
|
10 pg | 100 pg |
-
|
+
|
5 pg to 5 ng | - |
+
|
-
|
- | 5 pg to 5 ng |
-
|
+
|
- Positive detection (+) was defined as the presence of a peak in the melting profile which fulfills these conditions:
- (i) its melting point identified by thermocycler software lies within 91.0 ± 1.0°C for F. culmorum and 86.5 ± 1.0°C for F. graminearum
- (ii) absolute peak area amounts to at least 100 densitometry units
- (iii) the relative peak area in relation to the total area under the melting curve amounts to at least 10%.
- Identical results were obtained in the presence of an excess (10 ng) of wheat DNA.
