Table 2 Frequency of transgene insertion into sites other than attTn7. For each experiment shown, three independent electrotransformations of pGRG19 (carrying a ChlR transgene) into MG1655 and MG1655 attTn7::Tn7kan were done. The presence of the Tn7 at attTn7 prevents insertion events there and allows measurement of the frequency at which Tn7 can transpose to other sites as discussed in the text [13]. One single colony from each transformation was streaked out once at 32°C on LB with ampicillin. From this, a culture was grown overnight in LB at 32°C, and appropriate dilutions were plated at 42°C on LB and LB with chloramphenicol. Values shown are the frequency of ChlR
Strain | Experiment | Frequency of ChlR cells | Average ± SEM |
|---|---|---|---|
1 | 7.0 × 10-1 | ||
MG1655 | 2 | 7.2 × 10-1 | (7.9 ± 0.8) × 10-1 |
3 | 9.4 × 10-1 | ||
1 | 6.8 × 10-5 | ||
MG1655 attTn7::Tn7 | 2 | 4.6 × 10-5 | (5.5 ± 0.7) × 10-5 |
3 | 5.2 × 10-5 |