Table 4 Oligonucleotides used for IRS-PCR and plasmid-specific PCR.
Designation | Sequence | Used for | PCR conditions (°C/seconds) | No. of PCR cycles | Source | ||
|---|---|---|---|---|---|---|---|
| Â | Â | Â | Denaturation | Annealing | Extension | Â | Â |
Ps1 | 5'-GACTCGACTCGCATGCA-3' | Adapter 1, primer IRS-PCR | 94/30 | 55/60 then 60/30 | 72/90 | 5 then 30 | 30 |
AH2 | 5'-TGCGAGT-3' | Adapter 1 | Â | Â | Â | Â | 30 |
Sal1 | 5'-PO4-TCGATACTGGCAGACTCT-3' | Adapter 2 | Â | Â | Â | Â | 30 |
AX2 | 5'-GCCAGTA-3' | Adapter 2 | Â | Â | Â | Â | 30 |
PsalA | 5'-AGAGTCTGCCAGTATCGACA-3' | Primer IRS-PCR | 94/30 | 55/60 then 60/30 | 72/90 | 5 then 30 | 30 |
PsalC | 5'-AGAGTCTGCCAGTATCGACC-3' | Primer IRS-PCR | 94/30 | 55/60 then 60/30 | 72/90 | 5 then 30 | 30 |
PsalG | 5'-AGAGTCTGCCAGTATCGACG-3' | Primer IRS-PCR | 94/30 | 55/60 then 60/30 | 72/90 | 5 then 30 | 30 |
PsalT | 5'-AGAGTCTGCCAGTATCGACT-3' | Primer IRS-PCR | 94/30 | 55/60 then 60/30 | 72/90 | 5 then 30 | 30 |
CB5 | 5'-ATAATGAGATTAGAACAACCAAGA-3' | Primer QpH1 PCR | 94/120 | 53/60 | 72/120 | 35 | 56 |
CB6 | 5'-TCTTTCTTGTTCATTTTCTGAGTC-3' | Primer QpH1 PCR | 94/120 | 53/60 | 72/120 | 35 | 56 |
QpRS1 | 5'-CTCGTACCCAAAGACTATGAATATATCC-3' | Primer QpRS PCR | 94/60 | 54/60 | 72/120 | 36 | 56 |
QpRS2 | 5'-AACACCGATCAATGCGACTAGCCC-3' | Primer QpRS PCR | 94/60 | 54/60 | 72/120 | 36 | 56 |