Figure 2

Diagram of the bglP promoter region nucleotide sequence. The DNA sequence of bases 208 to 425 of the XbaI-Hind III restriction fragment displaying regulation promoter activity is shown. Boxes indicate the position of the putative -35, -10, and ribosome binding sites (RBS). The stem-arrow structure located above the DNA sequence (base 258) indicates the start site of transcription. The inverted repeat depicted by lines with diamond ends (located below bases 261–291) indicate bases of the RAT site that are involved with the formation of a hairpin structure in the mRNA in the presence of a beta-glucoside. The inverted repeat depicted by lines with ball ends (located below bases 281–361) indicate bases that are involved with the formation of the transcription terminator in the absence of inducing beta-glucoside. Note the overlapping nature of the two inverted repeat structures. The asterisk above base 366 indicates the last residue of the short terminated transcript in the absence of inducer. The start site of translation and the amino-terminal end of the bglP protein are also shown below base 420.