Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy

Table 1 Permeabilization protocols

	Incubation time (min)	Temperature (°C)	Permeabilisation
Triton X-100 (0.01%)	5	RT	-
	10	RT	-
Triton X-100 (0.1%)	5	RT	-
	10	RT	-
	20	RT	-
Lysozyme (2 mg ml^-1 in H₂O); Triton X-100 (0.1%, 5 min, RT)	30	RT	+
	30	37	++
	> 30	37	-^a
Lysozyme (2 mg ml^-1 in PBS); Triton X-100 (0.1%, 5 min, RT)	30	37	-
Lysozyme (2 mg ml^-1 in H₂O); Triton X-100 (0.1%, 10 min, RT);	30	37	-^a

ISSN: 1471-2180