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Figure 1 | BMC Microbiology

Figure 1

From: Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy

Figure 1

Fluorescence microscopy of M. tuberculosis H37Rv (pGFP22-4) hybridized with anti-GFP and anti-mouse coupled to Cy3 as primary and secondary antibodies. A) Without permeabilization treatment. B) After permeabilization with lysozyme (2 mg ml-1, 30 min, 37°C) and triton X-100 (0.1%, 5 min, RT). Left to right, DIC, Blue-2A, Cy3 and superposition of the fluorescent images. Bar = 3 μm.

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BMC Microbiology

ISSN: 1471-2180

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