Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barré syndrome by high-throughput AFLP analysis

Table 2 Putative GBS-markers detected by htAFLP

	strains^b						11168 hit sequence^d
marker^a	11168	GB11	cura 7	cura 276	O:41	length^c	begin	end	gene and function
1	-	+	+	+	+	112	324441	324363	Cj0355c, probable two-component regulator
2	+	-	-	-	-	265	400499	400264	Cj0432c, murD, probable UDP-N-acetylmuramoylalanine – D-glutamate ligase
3	-	+	+	+	+	115	575807	575893	Cj0615, pstA, probable phosphate transport system permease protein
4	-	+	+	+	+	152	576061	576182	Cj0615, pstA, probable phosphate transport system permease protein
5	-	+	+	+	+	198	904760	904594	Cj0967, possible periplasmic protein
6	+	-	-	-	-	253	904817	904596	Cj0967, possible periplasmic protein
7	-	+	+	+	+	241	1070741	1070662	Cj1136, probable galactosyltransferase
8	+	-	-	-	-	138	1073190	1073082	Cj1138, probable galactosyltransferase
9	-	+	+	+	+	97	1074261	1074320	Cj1139c, probable galactosyltransferase
10	+	-	-	-	-	169	1090751	1090612	Cj1157, dnaX, probable DNA polymerase III subunit gamma
11	+	-	-	-	-	89	1092245	1092185	Cj1161c, probable cation-transporting ATPase
12	+	-	-	-	-	181	1236007	1236080	Cj1306c, unknown
13	+	-	-	-	-	137	1236406	1236298	Cj1306c, unknown, similar to Cj1310c, Cj1305c, Cj1342c, Cj0617/0618
14	+	-	-	-	-	438	1264182	1264586	Cj1336, unknown, identical to Cj1318 (except the C-term)
15	+	-	-	-	-	163	1328311	1328184	Cj1394, probable fumarate lyase; Cj1393, metC', probable cystathionine beta-lyase
16	+	-	-	-	-	203	1334951	1334779	Cj1400c, fabI, probable enoyl- [acyl-carrier-protein] reductase [NADH]
17	+	-	-	-	-	102			Cj1101, probable ATP-dependent DNA helicase

^aThe individual markers have been given a numerical code. ^bThe plusses and minuses indicate the presence and absence, respectively, of the AFLP fragments. ^cThe estimated fragment lengths on the AFLP gels are given. ^dThe begin and the end positions in the NCTC 11168 genome are given for all BLAST hits, as well as the corresponding genes and their function. Note that markers 5 and 6 are largely overlapping and have a complementary pattern of presence and absence (see also Figure 1).

ISSN: 1471-2180