Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barré syndrome by high-throughput AFLP analysis

Table 1 Polymorphic htAFLP markers for NCTC11168 strains of diverse origin

	NCTC11168 strains^b							11168 hit sequence^d
marker^a	1	2	3	4	5	6	length^c	Begin	end	gene and function
1	+	+	+	+	-	+	469	28753	28317	Cj0023, purB, probable adenylosuccinate lyase
2	-	-	+	-	-	+	252	67665	67858	Cj0046, probable transmembrane transport protein pseudogene
3	-	-	+	-	-	-	110	122004	122083	Cj0117, pfs, probable 5'-methylthioadenosine\S-adenosylhomocysteine nucleosidase
4	-	-	+	+	+	+	118	153984	154075	Cj0150c, probable aminotransferase
5	-	-	+	+	+	+	86	212438	212383	Cj0227, argD, probable acetylornithine aminotransferase
6	+	+	+	+	+	-	420	573124	572971	Cj0612c, cft, probable ferritin; Cj0613, pstS, possible periplasmic phosphate binding protein
7	-	-	+	+	+	+	49	589048	589393	Cj0629, possible lipoprotein; Highly similar to Cj1678; Cj1678, possible lipoprotein
8	-	-	+	+	+	+	382	788562	788468	Cj0840c, fbp, probable fructose-1,6-bisphosphatase
9	-	-	+	-	-	-	463	1047891	1048322	Cj1116c, ftsH, probable membrane bound zinc metallopeptidase
10	-	-	-	+	+	-	228	1227296	1227125	Cj1295, unknown
11	+	-	-	+	-	-	226	1227296	1227117	Cj1295, unknown
12	+	+	-	+	+	+	191	1270291	1270131	Cj1339c, flaA, flagellin A
13	-	-	+	-	+	+	126	1510286	1510381	Cj1580c, probable peptide ABC-transport system ATP-binding protein

^aThe individual markers have been given a numerical code. ^bThe strains are numbered according to Additional file 1. The plusses and minuses indicate the presence and absence, respectively, of the AFLP fragments. ^cThe fragment lengths, based on fragment position in the AFLP gels. ^dThe begin and the end positions in the NCTC 11168 genome are given for all BLAST hits, as well as the corresponding genes and their function.

ISSN: 1471-2180