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Table 2 Consequences of selective manipulation of MSggN media composition on surface colony formation of the hag null strain. This data shows how systematically lowering concentrations of macronutrients and micronutrients affects the growth pattern on MSggN agarose plates. In each case 5 mM KCl was present, except where KCl was varied, and plates were inoculated in triplicate to reveal the surface colony pattern (film or tendrils) after 24 h growth at 37°C.

From: A defined medium to investigate sliding motility in a Bacillus subtilis flagella-less mutant

Media component 1X conc. 0.1X conc. 0.01X conc.
Macronutrients    
54 mM Glycerol Film Tendril Tendril
30 mM Na glutamate Film Tendril Weak growth
5 mM NaH2PO4 Film Tendril Tendril
Micronutrients    
5 mM KCl Film Tendril Tendril
2 mM MgCl2 Film Film Tendrila
700 μM CaCl2 Film Film Filmb
50 μM MnSO4 Film Film Filmc
50 μM FeSO4 Film Film Filmd
1 μM ZnSO4 Film Film Filmb
  1. aTendrils were also formed at the 10-3 to 10-5 dilutions.
  2. bFor these micronutrients, films were also formed at the 10-3 to 10-5 dilutions.
  3. cFilms were formed at the 10-3 dilution and tendrils were formed at the 10-4 and 10-5 dilutions.
  4. dTendrils were formed at the 10-3 to 10-5 dilutions.