Figure 1
From: A defined medium to investigate sliding motility in a Bacillus subtilis flagella-less mutant
Surface colonization by a B. subtilis hag mutant DS64 and its wild type parent (3610) on defined semi-solid media is dependent on the level of potassium ion and agarose concentration. Semi-solid MSggN agarose plates (0.3 to 0.6% w/v agarose) were inoculated in the center with sharpened toothpicks with both hag and 3610 strains (in triplicate). After growth for 18 h at 37°C, typical plates were photographed. Abbreviations: Mk, MSggN medium with 100 μM KCl; MK, MSggN medium with 5 mM KCl.