Figure 4
The scavenging of endogenously generated H 2 O 2 is a prerequisite for aerobic growth of H. influenzae Rd in chemically defined minimal medium. (A) Aerobic growth of wild-type H. influenzae Rd (closed diamonds) and hktE- pgdx- H. influenzae Rd (open triangles) in MIc medium. Aerobic growth of wild-type H. influenzae Rd (closed squares) and hktE- pgdx- H. influenzae Rd (open inverted triangles) in MIc medium enriched with a vitamin/amino acid supplement. (B) Anaerobic-to-aerobic shift (indicated by the single arrow) of a freshly diluted (1/50) wild-type H. influenzae Rd (closed diamonds) and hktE- pgdx- H. influenzae Rd culture (open triangles) in MIc medium and of a hktE- pgdx- H. influenzae Rd culture in MIc medium either supplemented with pyruvate (inverted open triangles) or catalase (open circles).