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Figure 2 | BMC Microbiology

Figure 2

From: Hydrogen peroxide scavenging is not a virulence determinant in the pathogenesis of Haemophilus influenzae type b strain Eagan

Figure 2

Both H. influenzae Rd and Hib Eagan deficient in both catalase (HktE)and PGdx can be regarded as totally hydroperoxidase-deficient since these double mutants lack the capability to remove low micromolar concentrations of H 2 O 2 from solution. To a phosphate-buffered solution of 1.5 μM of H2O2, 3 × 108 whole cells of exponentially growing wild-type H. influenzae Rd (solid diamonds), hktE- pgdx- H. influenzae Rd (open diamonds), wild-type Hib Eagan (solid triangles), or hktE- pgdx- Hib Eagan (open triangles) were added, after which the turnover of H2O2 was followed in function of time (see Methods). Mean values of triplicate experiments (± SEM) are shown.

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