Secondary structure: small subunit ribosomal RNA of E. sakazakii NCTC 11467. Nucleotides in green and blue represent primer sequence targets. Pink circles denote regions of importance as determined by Artificial Neural Networks. Nucleotide numbering follows the Reference Numbering System used for E. coli J01695 . Every 10th nucleotide is marked with a red tick mark and every 50th nucleotide is numbered. (Structure courtesy of Doug Smith, Accugenix, DE, USA).