Figure 5
From: Cyclic nucleotide specific phosphodiesterases of Leishmania major
Complementation of the PDE-deficient S. cerevisiae strain PP5. Panel A: plate without heat-shock treatment, incubated at 30°C for 18 h; panels B – D: plates heat-shocked for 15 min at 55°C, and subsequently incubated at 30°C for 18 h (panel B), 24 h (panel C) or 36 h (panel D). The plates always display rows of patches of two independent transformants transfected with the following plasmids: Row 1: pLT1 + TbrPDEA (= positive control); row 2: pLT1, empty plasmid (= negative control); row 3: pLT1 + LmjPDEA; row 4: pLT1 + LmjPDEB2; row 5: pLT1 + LmjPDEB1.