Table 1 Oligonucleotide primers used for module jumping and amplifications
Primer | Sequence (5'-3') | Use |
|---|---|---|
F_C3a | GCA(CT)CA(CT)AT(ACT)AT(ACT)TC(AGCT)GA(CT)GG(AGCT)TGG | Module jumping (C-A domain) |
R_T1a | C(AGT)A(GT)(AGT)A(AG)(AT)GA(AG)TG(ACT)CC(AC)CC | Module jumping (A-T domain) |
F_A3 | GG(ACT)(AT)C(AGT)AC(ACT)GG(ACT)(AC)A(AGCT)CC(ACT)AA(AG)GG | Module jumping (A-T domain) |
PL10987_R2 | CCGTTATCAGCAATAGTCCTA | Primer derived from pBc10987 |
PL10987_R4 | GAATATACTCAACAGTAGCTACC | Primer derived from pBc10987 |
PL10987_R5 | CGATACGTCAAATGTACTCGG | Primer derived from pBc10987 |
PL10987_R6 | GTGATTCATATTGTCTGGATACG | Primer derived from pBc10987 |
PL10987_F20 | GAAGAGAGATATGTCTGTACT | Primer derived from pBc10987 |
PL10987_F21 | GTATATTGTGGCAATTGATGAAGC | Primer derived from pBc10987 |