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Table 3 Occurrence of ces genes in B. cereus group members tested by hybridization assays. Several probes targeting all CDSs of the ces operon and CDSs from flanking regions (see Table 4) were used to test the distribution of ces genes in the B. cereus group.

From: Cereulide synthetase gene cluster from emetic Bacillus cereus: Structure and location on a mega virulence plasmid related to Bacillus anthracis toxin plasmid pXO1

Strainb

Subtypea

cesH

cesP

cesT

cesA1

cesA2

cesB1

cesB2

cesC

B. cereus

         

F4810/72

E

xc

x

x

x

x

x

x

x

MHI 1305

E

x

x

x

x

x

x

x

x

UHDAM IH41385

E

x

x

x

x

x

x

x

x

NVH 0075-95

EL

-

-

-

-

-

-

-

(x)

NVH 1519-00

EL

-

-

-

-

-

-

-

-

INRA C24

EL

-

-

-

-

-

-

-

-

F3003/73

EL

-

-

-

-

-

-

-

-

F4429/71

EL

-

-

-

-

-

-

-

-

NVH 200

EL

(x)

-

-

-

-

-

-

-

RIVM-BC-0063

EL

-

-

-

-

-

-

-

-

ATCC 10987

EL

-

-

-

-

-

-

-

-

WSBC 10892

EL

-

-

-

-

-

-

-

-

WSBC 10028

NE

-

-

-

-

-

-

(x)

(x)

WSBC 10035

NE

-

-

-

-

-

-

-

-

ATCC 14579T

NE

      

-

 

B. thuringiensis

         

WS 2620

NE

-

-

-

-

-

-

-

-

WS 2621

NE

-

-

-

-

-

-

-

-

WS 2632

NE

-

-

-

-

-

-

-

-

WSBC 28001

NE

-

-

-

-

-

-

-

-

WSBC 28002

NE

-

-

-

-

-

-

-

-

WSBC 28022

NE

-

-

-

-

-

-

 

-

WSBC 28023

NE

-

-

-

-

-

-

 

-

WSBC 28024

NE

-

-

-

-

-

-

 

-

B. mycoides

         

WSBC 10256

NE

-

-

-

-

-

-

-

-

WSBC 10257

NE

-

-

-

-

-

-

 

-

WSBC 10258

NE

-

-

-

-

-

-

 

-

WSBC 10276

NE

-

-

-

-

-

-

 

-

WSBC 10278

NE

-

-

-

-

-

-

 

-

WSBC 10292

NE

-

-

-

-

-

-

 

-

WSBC 10293

NE

-

-

-

-

-

-

-

-

WSBC 10360

NE

-

-

-

-

-

-

-

-

B. weihenstephanensis

         

WSBC 10001

NE

-

-

-

-

-

-

 

-

WSBC 10045

NE

-

-

-

-

-

-

 

-

WSBC 10202

NE

-

-

-

-

-

-

 

-

WSBC 10204T

NE

-

-

(x)

-

-

-

-

-

WSBC 10212

NE

-

-

-

-

-

-

 

-

WSBC 10296

NE

-

-

-

-

-

-

 

-

  1. a) B. cereus group members are classified as emetic (E), emetic-like (EL) and non-emetic (NE) according to [36]. b) for strain designation and details on strains tested see additional file Table S1; c) X hybridization observed; (X) weak hybridization signal; – no hybridization signal.