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Table 4 Comparison of microscopical and PCR-based determination of Coxiella cell numbers. The PCR measurements were performed in duplicate; mean values are shown.

From: Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii

Coxiella particles per μl
Determined microscopicallya Quantified by PCRb
4.2 × 106 5.00 × 106
4.2 × 105 3.05 × 105
4.2 × 104 3.15 × 104
4.2 × 103 2.45 × 103
4.2 × 102 1.45 × 102
4.2 × 101 1.70 × 101
4.2 × 100 0
  1. aCell numbers were only counted from undiluted sample.
  2. bReal-time PCR targeting the icd marker.