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Table 3 PCR quantification of DNA dilutions of the C. burnetii Nine Mile RSA493 strain. The measurements were performed in duplicate; mean values are shown.

From: Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii

  Calculated Valuesa Measured Valuesb  
DNA conc [pg/μl] icd IS1111 icd IS1111 IS per genomec
2900 1.3 × 106 2.6 × 107 4.5 × 106 5.9 × 107 13.1
290 1.3 × 105 2.6 × 106 5.2 × 105 8.1 × 106 15.6
29 1.3 × 104 2.6 × 105 3.9 × 104 5.0 × 105 12.8
2.9 1.3 × 103 2.6 × 104 2.8 × 103 3.5 × 104 12.5
0.29 1.3 × 102 2.6 × 103 2.3 × 102 2.6 × 103 11.3
2.9 × 10-2 1.3 × 101 2.6 × 102 1.5 × 101 2.6 × 102 17.3
  1. aNumber of target copies based on DNA concentration and genome length.
  2. bNumber of target copies based on PCR quantification using plasmid standards.
  3. cCalculated as IS1111 measured per icd measured.