Skip to main content

Table 2 Summary of eight different PCR runs performed on eight separate DNA dilution series of the C. burnetii Nine Mile RSA493 strain

From: Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii

icd marker IS1111 marker
No. of copies/μl Mean C t SDa CVb (%) No. of copies/μl Mean C t SDa CVb (%)
1.3 × 107 15.81 0.23 1.5 2.6 × 108 15.70 0.25 1.6
1.3 × 106 18.64 0.25 1.3 2.6 × 107 17.63 0.29 1.6
1.3 × 105 22.08 0.33 1.5 2.6 × 106 21.37 0.30 1.4
1.3 × 104 25.93 0.46 1.8 2.6 × 105 25.15 0.27 1.1
1.3 × 103 29.63 0.58 1.9 2.6 × 104 28.76 0.36 1.2
1.3 × 102 33.51 0.60 1.8 2.6 × 103 32.52 0.48 1.5
1.3 × 101 37.79 0.49 1.3 2.6 × 102 36.39 0.51 1.4
  1. aSD, standard deviation of eight replicates
  2. bCV, coefficient of variation