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Table 2 Summary of eight different PCR runs performed on eight separate DNA dilution series of the C. burnetii Nine Mile RSA493 strain

From: Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii

icd marker

IS1111 marker

No. of copies/μl

Mean C t

SDa

CVb (%)

No. of copies/μl

Mean C t

SDa

CVb (%)

1.3 × 107

15.81

0.23

1.5

2.6 × 108

15.70

0.25

1.6

1.3 × 106

18.64

0.25

1.3

2.6 × 107

17.63

0.29

1.6

1.3 × 105

22.08

0.33

1.5

2.6 × 106

21.37

0.30

1.4

1.3 × 104

25.93

0.46

1.8

2.6 × 105

25.15

0.27

1.1

1.3 × 103

29.63

0.58

1.9

2.6 × 104

28.76

0.36

1.2

1.3 × 102

33.51

0.60

1.8

2.6 × 103

32.52

0.48

1.5

1.3 × 101

37.79

0.49

1.3

2.6 × 102

36.39

0.51

1.4

  1. aSD, standard deviation of eight replicates
  2. bCV, coefficient of variation