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Table 1 Summary of heavy metal CuSO4 induction. Heavy metal CuSO4 induction: Summary of the growth of AN-T; PFU; PFU per 106A. nidulans; PFUs change rate for treated/control; PFU per 106A. nidulans treated/control and PIR with different concentrations of CuSO4 at 6 hours, 24 hours, and 48 hours after addition of different concentrations of CuSO4 at day 4 and non-CuSO4 added as a control.

From: Induction of temperate cyanophage AS-1 by heavy metal – copper

CuSO4

Control (No CuSO4 added)

Time after induction (hrs)

0

6

24

48

OD 750 nm

0.32 ± 0.03

0.39 ± 0.06

0.45 ± 0.03

0.56 ± 0.08

Cell numbers (107)

3.60 ± 0.28

2.60 ± 0.27

2.06 ± 0.36

3.57 ± 0.10

Plaque Forming Unit (PFU)

ND

353.00 ± 32.57

372.00 ± 16.97

479.00 ± 36.77

PFU per 106A. nidulans

ND

13.57 ± 0.23

18.37 ± 2.47

13.42 ± 0.65

CuSO4

3.1 × 10-4 M CuSO4

Time after induction (hrs)

0

6

24

48

OD 750 nm

0.32 ± 0.03

0.44 ± 0.05

0.34 ± 0.01

0.36 ± 0.03

Cell numbers (107)

3.60 ± 0.29

1.76 ± 0.14

1.12 ± 0.11

1.22 ± 0.03

Plaque Forming Unit (PFU)

ND

483.00 ± 18.39

160.00 ± 11.31

57.00 ± 9.90

PFU per 106A. nidulans

ND

27.44 ± 1.16

14.29 ± 0.44

4.67 ± 0.70

PFUs change rate for treatment/control

ND

1.37 ± 0.08

0.43 ± 0.01

0.12 ± 0.01

PFU per 106A. nidulans treated/control

ND

2.00

0.78

0.35

PIR (%)

ND

102.20 ± 5.19

-22.20 ± 12.60

-65.20 ± 3.65

CuSO4

3.1 × 10-5 M CuSO4

Time after induction (hrs)

0

6

24

48

OD 750 nm

0.32 ± 0.03

0.36 ± 0.03

0.41 ± 0.04

0.49 ± 0.01

Cell numbers (107)

3.60 ± 0.28

1.68 ± 0.14

1.62 ± 0.01

1.875 ± 0.11

Plaque Forming Unit (PFU)

ND

416.00 ± 8.49

345.00 ± 18.39

483.00 ± 9.90

PFU per 106A. nidulans

ND

24.83 ± 1.58

21.30 ± 1.24

25.76 ± 0.93

PFUs change rate for treatment/control

ND

1.18 ± 0.09

0.93 ± 0.01

1.01 ± 0.06

PFU per 106A. nidulans treated/control

ND

1.80

1.20

2.00

PIR (%)

ND

82.98 ± 8.61

15.95 ± 8.79

91.95 ± 17.32

CuSO4

3.1 × 10-6 M CuSO4

Time after induction (hrs)

0

6

24

48

OD 750 nm

0.32 ± 0.03

0.38 ± 0.09

0.44 ± 0.04

0.56 ± 0.01

Cell numbers (107)

3.60 ± 0.28

1.23 ± 0.08

1.81 ± 0.10

2.66 ± 0.13

Plaque Forming Unit (PFU)

ND

480.00 ± 25.46

526.00 ± 19.80

614.00 ± 5.66

PFU per 106A. nidulans

ND

39.18 ± 0.41

29.06 ± 0.50

24.14 ± 0.21

PFUs change rate for treatment/control

ND

1.40 ± 0.06

1.41 ± 0.01

1.28 ± 0.09

PFU per 106A. nidulans treated/control

ND

3.00

1.60

1.80

PIR (%)

ND

188.37 ± 1.80

58.19 ± 14.00

79.88 ± 10.68

  1. PIR – Percentage of Increase of Release. ND – Not Detectable.