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Table 1 Summary of heavy metal CuSO4 induction. Heavy metal CuSO4 induction: Summary of the growth of AN-T; PFU; PFU per 106A. nidulans; PFUs change rate for treated/control; PFU per 106A. nidulans treated/control and PIR with different concentrations of CuSO4 at 6 hours, 24 hours, and 48 hours after addition of different concentrations of CuSO4 at day 4 and non-CuSO4 added as a control.

From: Induction of temperate cyanophage AS-1 by heavy metal – copper

CuSO4 Control (No CuSO4 added)
Time after induction (hrs) 0 6 24 48
OD 750 nm 0.32 ± 0.03 0.39 ± 0.06 0.45 ± 0.03 0.56 ± 0.08
Cell numbers (107) 3.60 ± 0.28 2.60 ± 0.27 2.06 ± 0.36 3.57 ± 0.10
Plaque Forming Unit (PFU) ND 353.00 ± 32.57 372.00 ± 16.97 479.00 ± 36.77
PFU per 106A. nidulans ND 13.57 ± 0.23 18.37 ± 2.47 13.42 ± 0.65
CuSO4 3.1 × 10-4 M CuSO4
Time after induction (hrs) 0 6 24 48
OD 750 nm 0.32 ± 0.03 0.44 ± 0.05 0.34 ± 0.01 0.36 ± 0.03
Cell numbers (107) 3.60 ± 0.29 1.76 ± 0.14 1.12 ± 0.11 1.22 ± 0.03
Plaque Forming Unit (PFU) ND 483.00 ± 18.39 160.00 ± 11.31 57.00 ± 9.90
PFU per 106A. nidulans ND 27.44 ± 1.16 14.29 ± 0.44 4.67 ± 0.70
PFUs change rate for treatment/control ND 1.37 ± 0.08 0.43 ± 0.01 0.12 ± 0.01
PFU per 106A. nidulans treated/control ND 2.00 0.78 0.35
PIR (%) ND 102.20 ± 5.19 -22.20 ± 12.60 -65.20 ± 3.65
CuSO4 3.1 × 10-5 M CuSO4
Time after induction (hrs) 0 6 24 48
OD 750 nm 0.32 ± 0.03 0.36 ± 0.03 0.41 ± 0.04 0.49 ± 0.01
Cell numbers (107) 3.60 ± 0.28 1.68 ± 0.14 1.62 ± 0.01 1.875 ± 0.11
Plaque Forming Unit (PFU) ND 416.00 ± 8.49 345.00 ± 18.39 483.00 ± 9.90
PFU per 106A. nidulans ND 24.83 ± 1.58 21.30 ± 1.24 25.76 ± 0.93
PFUs change rate for treatment/control ND 1.18 ± 0.09 0.93 ± 0.01 1.01 ± 0.06
PFU per 106A. nidulans treated/control ND 1.80 1.20 2.00
PIR (%) ND 82.98 ± 8.61 15.95 ± 8.79 91.95 ± 17.32
CuSO4 3.1 × 10-6 M CuSO4
Time after induction (hrs) 0 6 24 48
OD 750 nm 0.32 ± 0.03 0.38 ± 0.09 0.44 ± 0.04 0.56 ± 0.01
Cell numbers (107) 3.60 ± 0.28 1.23 ± 0.08 1.81 ± 0.10 2.66 ± 0.13
Plaque Forming Unit (PFU) ND 480.00 ± 25.46 526.00 ± 19.80 614.00 ± 5.66
PFU per 106A. nidulans ND 39.18 ± 0.41 29.06 ± 0.50 24.14 ± 0.21
PFUs change rate for treatment/control ND 1.40 ± 0.06 1.41 ± 0.01 1.28 ± 0.09
PFU per 106A. nidulans treated/control ND 3.00 1.60 1.80
PIR (%) ND 188.37 ± 1.80 58.19 ± 14.00 79.88 ± 10.68
  1. PIR – Percentage of Increase of Release. ND – Not Detectable.