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Table 1 Target and non-target strains used in this study and results obtained with the chromogenic media DFI and ESIA, the α-glucosidase based PCR assay and the rRNA probe based VIT test.

From: Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection

Species (number of strains) Origin ESIA* DFI+ PCR VIT
E. cloacae (1) ATCC13047 neg neg neg neg
E. cloacae (1) LMG3008 neg neg neg neg
E. cloacae (6) clinical, cosmetics neg neg neg neg
E. cloacae (2) food neg neg neg neg
E. dissolvens (1) ATCC23373 neg neg neg neg
E. hermanii (1) wild strain neg neg neg neg
P. agglomerans (1) ATCC27155 neg neg neg neg
P. agglomerans (2) food, cosmetics neg neg neg neg
Pantoea sp. (1) food neg neg neg neg
K. oxytoca (1) cheese neg neg neg neg
S. liquefaciens (1) cosmetics neg neg neg neg
S. ficaria (1) production environment neg pos neg neg
Serratia sp. (1) cosmetics neg neg neg neg
Salmonella Enterititis (1) wild strain neg neg neg neg
S. sonnei (1) wild strain neg neg neg neg
E. coli (1) ATCC25922 neg neg neg neg
E. sakazakii (1) ATCC29544 pos pos pos pos
E. sakazakii (1) ATCC29004 pos pos pos pos
E. sakazakii (1) ATCC51329 pos pos pos pos
E. sakazakii (20) fruit powder1 pos pos pos pos
E. sakazakii (5) cosmetics pos pos pos pos
E. sakazakii (15) human2 pos pos pos pos
E. sakazakii (1) milk powder3 pos pos pos pos
E. sakazakii (2) baby food 4 pos pos pos pos
E. sakazakii (1) milk 5 pos pos pos pos
E. sakazakii (15) production environment6 pos pos pos pos
E. sakazakii (1) production environment pos neg pos pos
  1. * positive = turquoise colonies typical for E. sakazakii
  2. + positive = blue-green coloured colonies typical for E. sakazakii
  3. 1including strains (Acc. number): 858 (AY752936), 759 (AY752939), 954 (AY752938), AY752943
  4. 2including strains (Acc. number): ES 11 (AY803187), ES 4 (AY803186)
  5. 3including strain (Acc. number): FSM 265 (AY803191)
  6. 4including strain (Acc. number): FSM 393 (AY752941)
  7. 5including strain (Acc. number): FSM 33 (AY752940)
  8. 6including strains (Acc. number): FSM 468 (AY752942), FSM 266 (AY803190)