Table 5 E. coli strains and bacteriophage used in this study.

MG1655

Prototrophic E. coli K-12, F^- λ^-rph

CGSC, Yale University

GC4468

ΔlacU169 rpsL

GC122

as GC4468 but rpoS13::Tn10

[49]

HS1066

as GC4468 but argH⁺-λplac Mu53[Φ(argH-lacZ)66](rsd 1066)

[26]

HS1066p

as HS1066 but rpoS13:: Tn10

[26]

HS1072

as GC4468 but argH^--λplac Mu53[Φ(argH-lacZ)72](rsd 1072)

[26]

HS1072p

as HS1072 but rpoS13:: Tn10

[26]

HS2401

as MG1655 but ΔargR:: cat

This study

HS2402

as GC4468 but ΔargR:: cat

P1(HS2401)×GC4468→Cm^R

HS2403

as GC122 but ΔargR:: cat

P1(HS2401)×GC122→Cm^R

HS2404

as HS1066 but ΔargR:: cat

P1(HS2401)×HS1066→Cm^R

HS2405

as HS1066p but ΔargR:: cat

P1(HS2401)×HS1066p→Cm^R

HS2406

as HS1072 but ΔargR:: cat

P1(HS2401)×HS1072→Cm^R

HS2407

as HS1072p but ΔargR:: cat

P1(HS2401)×HS1072p→Cm^R

HS3006

As HS1066 but ΔastCADBE::cat

This study

HS3006p

As HS3006 but rpoS13:: Tn10

P1(HS3006)×HS1066p→Cm^R

B) Phage

P1vir

generalized transducing phage

Laboratory stock

C) Plasmids

pKD3

Template plasmid for gene disruption, cat is flanked by FRT sites.

[50]

pKD46

λ Red recombinase expression plasmid under control of an araC-P _araB inducible promoter.

[50]

pArgR

Derivative of pCA24N

H. Mori [31]